Chromotography

-> Used to separate mixtures of substances into their components
-> Stationary phase: a solid, or a liquid supported on a solid
-> Mobile phase: A liquid or a gas
     => Flows through the stationary phase and carried the components of the mixture with in. Different components travel at different rates.

Retention Factor - Rf
- Unknown substances separated by chromatography can be identify by Rf values
- Rf value = Distance move by substance/distance move by the solvent

Uses
- Separate and identify compounds in a mixture
- For colourless compounds, at the end of the experiment, the chromatogram is sprayed with a locating agent
- The locating agent is a chemical substance that will produce colored products on reaction with colorless compounds

Paper Chromatography
- Stationary phase: Uniform absorbent paper
- Mobile phase: Suitable liquid solvent or mixtures of solvent
- Separate a mixture of solutes with different solubility and degree of absoption