Monday, 5 September 2011

DNA testing

1. hydrogen peroxide

 Top right: sample 1, Top middle: sample 2, Top left: sample 3, bottom right: sample 4, Bottom middle: sample 5, Bottom left: sample 6
After adding hydrogen peroxide, samples 4, 5 and 6 bubbled.

Results: 
Sample 1
My prediction: May be blood -> Red 
Observation: No bubbles
Result: Negative 

Sample 2
My prediction: May not be blood -> Orange
Observation: No bubbles
Result: Negative

Sample 3
My prediction: May not be blood -> Pink 
Observation: No bubbles
Result: Negative

Sample 4
My prediction: May be blood -> Red and quite thick
Observation: Bubbles!
Result: Positive

Sample 5
My prediction: May not be blood -> Purple 
Observation: Bubbles!
Result: Positive

Sample 6
My prediction: May not be blood -> bright red with small particles flatting around 
Observation: Bubbles!
Result: Positive

Conclusion: Samples 4, 5 and 6 may contain blood as hydrogen peroxide reacts with blood and bubbles. 

2. Luminol testing
 Spray Luminol on all three samples
 Notice sample 4(one on the left), it glows
Notice that sample 5 also changes colour
 Notice that the colour of all three samples are different

Results: 
Sample 4: Glowed when luminol was applied
Sample 5: Glowed when luminol was applied
Sample 6: Didn't glow

Conclusion: Sample 4 and 5 may be blood as Luminol chemiluminescence can react with a number of substances such as copper or copper-containing alloys, ; and, as a result, producing the typical blue glow

3. Kastle Meyer Reagent 
 Before adding the reagent 
 After adding reagent

Results: 
Sample 4: Changed colour -> pink
Sample 5: No change
Conclusion: Sample 4is blood


DNA extraction
We also extracted DNA from our cheek cells but unfortunately, i didn't take any pictures. 


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